Improved Methodology for Isolating Soil Microorganisms
نویسندگان
چکیده
منابع مشابه
Transport of Microorganisms through Soil
Microorganisms migrating into and through soil from sources on the land surface may cause a serious threat to both ground and surface waters. It has been estimated that microorganisms can migrate significant distances in the field. Results from various studies suggested that preferential flow through macropores, worm holes, cracks, and fractures is the main reason for such observations. However...
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Ethylene-producing strains of Penicillium cyclopium and P. crustosum were isolated from soil. These isolates produced ethylene on a variety of carbon growth substrates including phenolic acids. The quantities of ethylene produced on the various substrates varied, and the subtrate-ethylene prosuction pattern for P. cyclopium strains differed significantly from that of P. crustosum strains.
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A selective medium was developed that is capable of isolating Phanerochaete chrysosporium from soil. This medium contains 15 ppm of benomyl (15 mug g) and 550 ppm of streptomycin sulfate in 2% malt agar and is held at 39 degrees C after inoculation. P. chrysosporium was isolated from three nonsterile forest soils to which the fungus had been added. These soils contained large microbial populati...
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Urine was collected from newborns, children, and adults who had received a tracer dose of [3H] cortisol. The free and different types of conjugated cortisol metabolites were separated by ion-exchange chromatography. A modified Amberlite XAD-2 procedure was used, which enabled quantitative extraction of the extremely polar cortisol metabolites from urine from newborns. Large differences between ...
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In biological control studies of plant-parasitic nematodes in field soil improved methods are needed for reducing or eliminating specific soil inhabiting microorganisms. Microwave heating of soil decreases soilborne fungi and bacteria, but not Pasteuria spp. Bacterial and fungal colony forming units were reduced to nondetectable levels in microwaved heated field soft (650 watts) at 5.2% moistur...
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ژورنال
عنوان ژورنال: Applied Microbiology
سال: 1970
ISSN: 0003-6919
DOI: 10.1128/aem.19.1.186-187.1970